Enzyme Trypsin Serine Lysine Protease Arginine Protease Recombinant
Trypsin can autocatalytically activate more trypsinogen to trypsin.
Trypsin consists of a single chain polypeptide of 223 amino acid
residues. This native form of trypsin is refered to as β-trypsin.
of β-trypsin (which is cleaved at Lys- Ser in the bovine sequence) results in α-trypsin which is held together
by disulfide bridges. Trypsin is a member of the serine protease S1
family. The active site
amino acid residues of trypsin include His and Ser
Trypsin is a member of the serine protease family. Trypsin cleaves
peptides on the C-terminal
end of lysine and arginine amino acid residues. The optimum pH of
trypsin is pH 7 - 10. The
enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by
metal chelating agents,
(1)Animal origin free: recombinant trypsin is no exogenous virus
contamination,and any animal origin material is not used in the
(2)Stable quality: Mass production can ensure stable and continuous
batch production.It is no difference between the batch and the
product quality is stable.
(3)High purity: Higher specific activity.Host protein residues is
less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is
easy to store and transport.
(5)Compliance with regulatory requirements: Production equipment
and production environment comply with relevant regulatory
requirements, and the production process is in full compliance with
NSF ISO 9001: 2008 quality system and GMP guidelines.
(6) Complete quality documents: we can provide relevant regular
support files in according to customers’ requirement.
NLT 70% β-trysin, NMT 20% α-trypsin
No chymotrypsin, carboxypeptidase A, and other protease
One USP unit of trypsin activity will produce a Delta A253 of 0.003
per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with
BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to
aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin