Recombinant Trypsin Lyophilized Powder Trypsin for Mass
Trypsin is a member of the serine protease family. Trypsin cleaves
peptides on the C-terminal end
of lysine and arginine amino acid residues. The optimum pH of
trypsin is pH 7 - 10. The enzyme is inhibited by serine protease
inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein
expressed in E.coli and purified
by high pressure liquid chromatography. There are no contaminating
enzyme activities such as carboxypeptidase A and chymotrypsin. No
protease inhibitors such as PMSF are contained in the preparation.
Animal origin free:The use of recombinant Porcine Trypsin
eliminates the risk of virus presence,
and other potential adventitious agents found in animal-derived
trypsin. YaxinBio Recombinant
Porcine Trypsin belongs to the AOF level 3.
Stablility:A sterile recombinant trypsin lyophilized powder
eliminates the contamination risks and decreases the chance of
activity loss in the process of transport and storage.
1) Recombinant porcine trypsin provides increased specific activity
and eliminates contaminating proteases activities found in
2) No other contaminating proteases such as chymotrypsin or
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥3800 USP units/mg pro.|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||24.0±2.4 kDa|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and
25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to
aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in
sealed container. It is stable
within 24 months.After dissolved, it should be stored under -20℃.
It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and