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E.coli Recombinant Trypsin Animal Origin Free Trypsin 3800 USP u/mg Pro

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E.coli Recombinant Trypsin Animal Origin Free Trypsin 3800 USP u/mg Pro

Brand Name : YaxinBio
Model Number : RPT0201
Certification : ISO 9001 2015
Place of Origin : China
MOQ : 10mg
Price : 65$/10mg
Payment Terms : T/T
Packaging Details : ice packaging
Supply Ability : 1000g per week
Product : Recombinant Porcine Trypsin
Physical form : White lyophilized powder
Specific activity : ≥3800 USP u/mg pro
Protein content : 35% ~ 70%
Related Product : Sequencing Grade Trypsin
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E.coli Recombinant Trypsin Animal Origin Free Trypsin 3800 USP u/mg Pro


Description


Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,e.g.EDTA.


Assay Method
The activity of most Sigma preparations is determined by a continuous rate spectrophotometric assay

and expressed in BAEE units.

Unit Definition: One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).


Conditions
Temp = 25 °C, pH = 7.6, A253nm, Light path = 1 cm
In a 3.2 ml reaction mix, the final concentrations are 63 mM sodium phosphate, 0.23 mM Nα-benzoyl-L-arginine ethyl ester, 0.06 mM hydrochloric acid, and 100 units trypsin.


Unit Conversions

1 BAEE µM Unit = 200 BAEE Units
1 TAME µM Unit = 0.27 BAEE µM Units
1 BAEE µM Unit = 3.64 TAME Units
1 TAME µM Unit = 55 BAEE A253 Units
1 BAEE A253 Unit = 0.018 TAME µM Unit
1 TAME µM Unit = 180 TAME A247 Units
1 TAME A247 Unit = 0.33 BAEE Units
1 USP Unit = 3.0 BAEE Units
1 NF Unit = 1.1 USP Units


Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.


Advantages

Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

and other potential adventitious agents found in animal-derived trypsin.

YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.


Main Features


Source

E. Coli

Purified by

HPLC

Purity(RP-HPLC)

NLT 70% β-trysin, NMT 20% α-trypsin

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.


Recommend Usage


Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50

to 1:1000 (w/w).The optimum pH is pH7-10.


Storage Instructions


Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is

stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within

24 months and above 90% activity remained after 10 times repeated freezing and thawing.



Product Tags:

porcine trypsin

      

cell culture trypsin

      
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