Sign In | Join Free | My pysqlite.org
pysqlite.org
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd.
Home > Recombinant Trypsin >

Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease

Shanghai Yaxin Biotechnology Co.,Ltd.
Trust Seal
Verified Supplier
Credit Check
Supplier Assessment

Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease

Brand Name : YaxinBio
Model Number : RPT0201
Certification : ISO 9001 2015
Place of Origin : China
MOQ : 10mg
Price : 65$/10mg
Payment Terms : T/T
Supply Ability : 100000g per week
Packaging Details : ice packaging
Delivery Time : 7 days
Product : Recombinant Porcine Trypsin
Cat. No. : RPT0201
CAS : 9002-07-7
EC : 3.4.21.4
Related Product : Recombinant Carboxypeptidase B
White lyophilized : HPLC
Contact Now

Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease


Recombinant Trypsin

Description


Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal

end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The

enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,

e.g.EDTA.Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli

and purified by high pressure liquid chromatography. There are no contaminating enzyme

activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF

are contained in the preparation.

Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid residues.

The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and no

cleavage occurs if a proline residue is on the carboxyl side of the cleavage site.

Trypsin will hydrolyze ester and amide linkages of several synthetic substrates.


Advantages


Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant

Porcine Trypsin belongs to the AOF level 3.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.


Storage Instructions


Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months

and above 90% activity remained after 10 times repeated freezing and thawing.


Main Features


Source

E. Coli

Format

White lyophilized

Specific activity

≥3800 USP units/mg pro

Purity(RP-HPLC)

NLT 70% β-trysin, NMT 20% α-trypsin

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.


Unit Dediniton


One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


Recommend Usage


Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is 7-10.



Product Tags:

porcine trypsin

      

cell culture trypsin

      
China Customized Inflatable Bumper Ball Game Bubble Adult Grass CE supplier

Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease Images

Inquiry Cart 0

Home| Products| Suppliers| Quality Suppliers| Site Map |About Us |Contact Us |Help |关于我们 |联系我们

Copyright © 2009 - 2018 pysqlite.org. All rights reserved.