Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine
Trypsin is a member of the serine protease family. Trypsin cleaves
peptides on the C-terminal
end of lysine and arginine amino acid residues. The optimum pH of
trypsin is pH 7 - 10. The
enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by
metal chelating agents,
e.g.EDTA.Recombinant Porcine Trypsin is a genetically engineered protein
expressed in E.coli
and purified by high pressure liquid chromatography. There are no
activities such as carboxypeptidase A and chymotrypsin. No protease
inhibitors such as PMSF
are contained in the preparation.
Trypsin will cleave peptides on the C-terminal side of lysine and
arginine amino acid residues.
The rate of hydrolysis is slower if an acidic residue is on either
side of the cleavage site and no
cleavage occurs if a proline residue is on the carboxyl side of the
Trypsin will hydrolyze ester and amide linkages of several
Stablility:A sterile recombinant trypsin lyophilized powder
eliminates the contamination risks and decreases the chance of
activity loss in the process of transport and storage.
Animal origin free:The use of recombinant Porcine Trypsin
eliminates the risk of virus presence,
and other potential adventitious agents found in animal-derived
trypsin. YaxinBio Recombinant
Porcine Trypsin belongs to the AOF level 3.
1) Recombinant porcine trypsin provides increased specific activity
and eliminates contaminating proteases activities found in
2) No other contaminating proteases such as chymotrypsin or
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in
sealed container. It is stable
within 24 months.After dissolved, it should be stored under -20℃.
It is stable within 24 months
and above 90% activity remained after 10 times repeated freezing
≥3800 USP units/mg pro
NLT 70% β-trysin, NMT 20% α-trypsin
No chymotrypsin, carboxypeptidase A, and other protease
One USP unit of trypsin activity will produce a Delta A253 of 0.003
per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a
substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to
aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is 7-10.